Identification of leaf proteins differentially accumulated during the overwintering in frost-tolerant winter wheat

Mohsen Janmohammadi


Overwintering crops, such as winter wheat, suffer a lot of changes in their proteome during a period of cold acclimation and during the phase transition from vegetative to floral growth. Phase transition is an important character for winter wheat under cold region because it decreases the ability of wheat plants to maintain frost tolerance. Reproductive development in winter wheat is triggered by fulfilment of vernalization requirements. In order to gain insight into the molecular changes at the protein level in hardy winter wheat (cv. Norstar) during the overwintering we applied a 2-DE based proteomic approach. Plants were grown under under field condition in cold region (Zanjan). Proteins were extracted from the leaves collected from plants on tree different growth stages include of T1: vegetative growth when plant increased their frost tolerance (23-Nov, LT50: -12 C), T2: vernalization saturation point when plants acquired to maximum frost tolerance (21-Dec, LT50: -28 C) and T3: after vegetative/reproductive Phase transition (21-Feb, LT50: -16 C). Approximately 1000 protein spots were separated and visualized on CBB-stained 2-D gels. 110 protein spots were found to be up-regulated in responding to transition from T1 to T2 stage and 34 proteins had been down-regulated. Spots comparison between T3 and T2 revealed that 160 spots were responsive which 76% had been up-regulated. Expression profile of some identified proteins like as Cell division control protein, asparagine synthase, Translational elongation factor, ribulose-1,5-bisphosphate carboxylase activase, actin, harpin binding protein and heat shock protein 70 during the investigated stages nearly were similar to LT tolerance trend. This study indicates that accumulation of some identified protein spots like as ATP synthase, fructose bisphosphat aldolase, adenosine kinase like protein and phosoribulokinase-like protein 2 were increased more than 2-fold, on the contrary expression of some protein such as boron transporter, heat shock 70 and RuBisCO activase intensively decreased during vegetative/reproductive transition.

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